ear tagging mice protocol

Ear tagging involves using a special applicator to place an "earring," or metal tag with an identification number, in the pinna of a mouse's ear. Ear Tags Tags must be appropriately size for species and age. The tattoo is placed on the tail, toe or ear. Certain experimental protocols require genotyping of neonates as early as day one, which makes it essential that these pups be permanently identified. To circumvent this problem, a secondary form of identification such as a punch code on the ear opposite of the tag should be utilized. Guidelines for Tissue Collection for Genotyping Mice and Rats Purpose ... Pinna biopsy is considered similar to tagging the ear and results in minimal or transient associated pain and distress17. And holes in both indicate 300. UID microchips are MRI, Gamma/X-Ray and Ultrasound compatible. Subcutaneously implanting a microchip is another method of permanently identifying a mouse. Be sure to order the applier that is appropriate for the specific size and style of ear tags being used. Slip the tag out so that the crimped end does not pass through causing trauma and enlargement of the piercing hole. Again, clean the punch with a disinfectant, rinse with alcohol and dry it thoroughly to prevent rusting. Following application, we will also discuss removal of the ear tag, which is usually performed in case of an infection due to the tag application. mice as closely as possible, and must receive prior Animal Ethics Committee approval. Ergonomically, the lever punch is easier to use than thumb punch, especially for the thicker ears of the older rats. New York, New York: Dover Publications; 706 p. Ingalis, JK. Different investigators use various methods to identify their mice. The same procedure is applicable to any other code or punch. Several systems are available for individually identifying research mice. Other companies may also sell them. 1. Then, firmly squeeze the applier closed; the ear tag will pierce the ear and lock together. Experimental protocols often require mice to undergo repeated procedures that can cause pain or discomfort. Another limitation is that most animals must be grasped at the scruff in order to read the code, thus increasing the times they are handled. Use of „by-products‟ from these techniques to provide material . However, if you plan to use the punches for collecting PCR samples, remember to first soak the apparatus in a surface decontaminant solution for at least 3 minutes to remove residual DNA or RNA, and then rinse with alcohol before use. Then, release the punch and remove it from the ear. Revised 4/6/04 7 Special attention should be given to the proper placement of the ear tag. In order to remove the tag, moisten a cotton-tipped applicator with an antiseptic solution and use it to remove any debris from around the tag. AALAS training manual series, vol 1: Assistant laboratory animal technician. 1975. Guidelines for Genotyping Laboratory Mice and Rats. First, the ear punch code discussed above has an upper limit of 399, which may limit the use of this method in some large-scale studies. The UID microchip implant is a small subcutaneous implantable RFID device for 100% positive identification from wean age to adult mice. Next, in order to quantify cancer progression, they imaged the animals using bioluminescence at one, ten and eighteen days post injection. The ear tag should not be applied until the day of weaning. Methods for permanently identifying mice include ear punching, ear tagging, tattooing, and subcutaneously implanting a numerically coded microchip. Male and female BALB/c mice … Tags should be properly placed to avoid irritation or trauma. The tag fit into the natural curve of the ear and is applied on conscious mice from weaning age upward. The JoVE video player is compatible with HTML5 and Adobe Flash. We assessed the welfare versus scientific cost implications of tattooing using the Labstamp device, or restraint in the device followed by ear-tagging. Ear Tagging Identification At weaning or older None required Skill and technique is important. Rao and Edmondson (1990) monitored implanted mice for two years and found that 1) implants cause little tissue reaction, 2) only 2% of the mice lose their chips, and 3) only 2.8% of the chips fail. Tattooing Tattooing is a means of permanent identification. The tagging system requires the application of two identically numbered yellow plastic ear tags to calves born on a holding within 20 days of birth. Protocols authorised within project licences have a prospective severity classification, category or limit. Now, let's move on to ear tagging, which is another inexpensive and easy-to-perform method for the individual identification of mice and rats. Now, let's move on to ear tagging, which is another inexpensive and easy-to-perform method for the individual identification of mice and rats. In: Green EL, editor. • Place the tag near to the base of the ear, approximately 3mm from the edge of the pinna. Use pigment approved by the United States Food and Drug Administration. Tags must be placed such that they do not cause a bend in the ear, interfere with the animal's mobility, or catch on any part of the caging. Here, we will use a metal tag to demonstrate the procedure on mice. Ear tagging and ear marking are used to identify livestock. Then, using wire cutters, cut across the top of the tag loop from edge to edge. To begin, decide the animal's experimental code and the punch to be used. Here, we are going to discuss the two most commonly used methods: ear tagging and ear punching. Importantly, do not cut across the flat surface as that will compress the tag, pinch the ear, and cause damage to the ear. In severe cases of infection, the animal may need to be anesthetized. Ear tagging is perceived as less painful or stressful than tattooing and therefore is generally considered less harmful or costly to welfare. You should now understand the ear punch code, know the different types of commercially available rodent ear tags, and should be able to accurately perform these procedures without causing significant animal discomfort. Depending on your mouse identification needs and the degree of visibility you require, you can choose from several varieties of mice ear tags, including stamped, paint-filled stamped, and laser etched mouse ear tags with numbers on either … The codes are easily distinguished from one another by a computer. Call 859-261-2035. If that doesn't help, please let us know. Please check your Internet connection and reload this page. Once the animal is restrained, position the point of the ear tag deeply in the concha of the ear so the tag numbers face the back of the animal. Ear tagging requires a special applicator to place an “earring” or metal tag with an identification number in the pinna of a mouse or rat’s ear. Ear tagging involves using a special applicator to place an "earring," or metal tag with an identification number, in the pinna of a mouse's ear. Tennessee: Cordova; 186 p. We use cookies to personalize our website and to analyze web traffic to improve the user experience. In addition to considering cost, the ease of the technique and the level of pain to the animals should be some of the primary considerations when choosing the appropriate identification method. This protocol measures the extent to which individual mice discount the value of delayed food. The scissor punch does not require a pinching motion, thus avoiding carpal tunnel fatigue and injury. Non-toxic markers: Sharpies can be used to mark the tail or fur of rodents, however the mark must be reapplied every 24 hours to ensure the mark is still visible. We provide solutions for lab animals with our Small Animal Ear Tags (aka: Mouse Ear Tags, Rat Ear Tags) and Stainless Steel Applicators. Other number sequences available by special order. Policy: Toe clipping for the purposes of identification and/or genotyping may be approved in the animal protocol under the following circumstances: While the tags provide an individual identifier for a mouse or rat, they are not infallible. Before starting the procedure, it is recommended that punching tools be cleaned with a disinfectant solution,rinsed with alcohol, and dried thoroughly. Risk of infection or tag falling out. A. Before using an ear punch, it should be tested to make sure it works properly and the cutting edge should be disinfected. A JoVE representative will be in touch with you shortly. Ear tagging: A metal tag with a unique identification number is attached to one ear of the rodent. Now let's learn how to properly apply an ear tag, as incorrect positioning can have several unfortunate consequences. 4.2.3. The tattoo is placed on a toe or tail. Because the implants are relatively large, they are not recommended for newborns, but they are acceptable for identifying weanlings and adults. Ear tagging. The description includes training steps, which may require an average of 12-29 sessions depending on the mouse strain, and an experimental phase in which delay discounting is assessed over 5-10 weeks. By continuing to use our website or clicking “Continue”, you are agreeing to accept our cookies. To learn more about our GDPR policies click here. B. Ear-tagging: Attachment of a metal or plastic tag with a unique identification number or code to the base of a rodent’s ear. Mice do not need to be anesthetized to be ear-punched. Older mice are either placed in a restraining device or sedated. 3. A subscription to JoVE is required to view this content.You will only be able to see the first 20 seconds. ear punch, tag, microchip) are not appropriate. If several animals are housed in the same cage, the loss of an ear tag can be troublesome. Tattoos may also be used to mark weanling and older mice. A facility should standardize both the choice of ear to be tagged and the code to be used on the other ear, and all codes should be indicated on the cage card. FDA approved tattoo pigment must be used. We use/store this info to ensure you have proper access and that your account is secure. If the tag is placed too close to the edge of the ear, it can easily be torn. Let's use the code 173 and scissor punch as examples here. The rational for this is that toes can be clipped anytime from P7 until around 13 days of age Dickie, MM. There are a variety of commercially available ear tags. When you release, the tagged ear will fall out of the applier. If positioned too close to the head, the skin of the neck area can catch in the tag, creating irritation, pain, and restricted movement, which can then lead to self-trauma. Ear Tags Ear tags are another means for identifying mice. The use of toe tattooing allows such animals to be identified until they are large enough for ear tagging or ear punching. Animal Marker is another product available which can be used on rodent’s fur. After the dark period (16–24 h), the mean MAP was 102.6 (0.5) mmHg after the ear tattoo, 102.9 (0.4) mmHg after the ear notching and 101.2 (0.5) mmHg after the microtattoo. FDA-approved pigment on the tail, toe or ear to identify mice of any age. All rights reserved. In spite of being a quick and simple method of identification, ear punching does have some limitations. Place the right ear in the punch so that the hole of the punch is positioned away from the pinna margin. If the problem continues, please. Used to deliver personalized information and tailor communications. The thumb punch requires a pinching motion, but is small enough to fit into an Eppendorf tube so that the ear tissue can be more easily collected. Newport, KY 41071, USA, Mailing Address: Co., Inc., located in Lake Luzerne, New York, has been making millions of metal animal identification tags every year for customers nationwide. 1990. While being tattooed, neonatal mice are manually restrained. Ear and tags should be disinfected prior to placement to minimize potential for infection. Supplied in packages of 500 numbered 001 to 500 or 501 to 1000. You may decline these cookies although certain areas of the site may not function without them. Confirm that the correct code was punched before adding the animal back to the cage. Before being implanted, mice are either sedated or anesthetized. Now that you have some background information about the ear punch code and the types of tools available, let's learn how to perform this technique in mice. Repeat the same procedure on the other ear to punch three notches - two for "20" near the outer corner and one for "50" near the inner corner, totaling the code to "70". Holes in the center of the right and left pinna represent 100 and 200, respectively. At this time, the animal should be identified by an appropriate method (ear tag, transponder, ear punch,…) 6 Tissue reaction to an implantable identification device in mice. Animals may be less likely to struggle if the hind limbs can rest on a surface such as the cage top or a counter top. Ear Tagging Vs Notching/ Puncturing. The punched-out ear tissue may be sufficient to perform PCR genotyping. I. Avoid autoclaving the ear punches, as it will eventually dull them. Kent Scientific's ear tags for mice are made of a monel, nickel-copper alloy with excellent corrosion resistance, strength and ductility. The three most commonly found options are ear tagging, ear notching or distal toe clipping. Ear tags can be imprinted by the manufacturer with several digits or letters. The Applicator is sold separately. • Place tag appropriately into applicator device. These include: metal tags that have a series of numbers and/or letters etched on them, and plastic tags with characters or barcodes on brightly colored backgrounds. Since tags are relatively large and mouse ears develop quickly, ear tagging is primarily used on weanling and older mice. • Soak ear tags in alcohol or other disinfectant prior to application. We use distal toe clipping. 14 days or older No anesthesia is required when performed by trained personnel Ear tagging can lead to pressure necrosis, ulcerations, inflammation, neoplasia and infection. Animals must be 14 days or older. Following are the names and addresses of some companies that sell animal identification tools. 2. However, ear tags are more difficult to read than tattoos and can fall out, and mice usually require restraint for … One disadvantage of metal tags is that it is often necessary to pick up the animal in order to read the code. Identification technique's importance increases further if the experiment is lasting over several days. UID Microchip Implant. Tattooing is the preferred method for identifying neonatal mice. A two (2) millimeter ear punch or marginal notch is recommended. A patch of fur on the back or side of the mouse may be shaved; such marks generally last 1-4 weeks (depending on stage of the hair cycle) and can be used on any color mouse. Proper identification of lab animals is a fundamental requirement of biomedical research. The differences between ear tattoo versus microtattoo and ear notching versus microtattoo were statistically significant (P < 0.05) (Figure 4 and Table 1). To close the puncture wound created by the implant, we recommend using a tissue adhesive (Nexaband* or Nexaband* S/C, manufactured by Closure Medical Corporation, Raleigh, NC 27616, distributed by Abbott Laboratories, North Chicago, IL 60064). As you might have guessed, ear punching involves punching a hole or a notch in the animal's external ear or pinna. An ear tag is a plastic or metal object used for identification of domestic livestock and other animals. Ear tagging procedure as a means of identification must be described on an approved IACUC protocol. When you are ready to apply the ear tag, restrain the animal so that the ears are easily accessible. If the ear tag uses Radio Frequency Identification Device technology it is referred to as an electronic ear tag.Electronic ear tags conform to international standards ISO 11784 and ISO 11785 working at 134.2 kHz, as well as ISO/IEC 18000-6C operating in the UHF spectrum. WEB Administration Of Drugs and Experimental Compounds in Mice and Rats Updated January 1, 2014 PDF Animal Care Consent to Treat Updated July 26, 2016 PDF Animal Care Med Campus Animal Order Form Updated May 21, 2020 • Gently restrain mouse with gloved hands by scruffing the dorsum high on the neck to control both the body and the head. Ear punching of weaning mice does not require anesthesia. Introduction to Laboratory Animal Science and Technology. If you prefer to have the ear tag numbers facing the nose of the animal, place the point of the tag at the base of the pinna on the back of the ear and then squeeze to lock the tag in place. Your access has now expired. Tags can be lost due to over-grooming, dermatitis or infection, self-trauma inflicted trying to remove tags due to intolerance, fighting, and aggressive breeding behavior. To make a notch, place the punch on the edge of the ear pinna at the correct spot, in this case at the top of the right ear for the "three" in the code's unit place, and do the same - apply quick and firm pressure and then open the scissor to remove it. One major advantage of using ear punch code is that the punches can double as tissue samples for genotyping, thus reducing stress to the animals caused due to repeat handling. New York, New York: Pergamon Press; 323 p. Rao GH, J Edmondson. Improperly placed ear tags can become easily detached from the ear. See more video protocols, please visit http://www.video-protocols.com Food coloring may be used to dye a patch of fur; such marks generally last for 1-2 weeks but can be used only on albino and light-colored mice. It also has business benefits by enabling livestock to be identified on-farm, leading to improved management. If there is a purulent discharge from the ear, follow-up treatment may be necessary and consultation with the veterinary staff for assessment is recommended. 1991. Ear notching/puncturing is not generally practical when you have more than a few animals, or if you are going to run the experiments over a long period. Mice do not need to be anesthetized to be ear-punched. The holes and notches are placed according to a predetermined code (Dickie 1975; Ingalis 1980; Stark and Ostrow 1991). Ear punching can be done at age 14 days or older without anesthesia. Avoid pulling or twisting the punch to prevent tearing the ear. Caution should be used to avoid cross-contamination of samples. We recommend downloading the newest version of Flash here, but we support all versions 10 and above. Microchips are about the size of a grain of rice and transmit a unique alphanumeric code for each mouse. Therefore, it is best to perform the ear punches on young rats as they are weaned. 2. In contrast, plastic tags are easy to read without having to handle the animals. Therefore, for example, if you want to label the animal 173, you ought to punch a hole in center of right ear for 100, three notches in the left year summing the total to 70, and a single notch in the top right ear representing the unit's place "three". You've just watched JoVE's presentation demonstrating the two commonly used methods of identification in rodents: ear tags and ear punches. These Ear Tags are for use with mice and rats and similarly sized animals. These include: metal tags that have a series of numbers and/or letters etched on them, and plastic tags with characters or barcodes on brightly colored backgrounds. When you are ready, restrain the animal by grasping it at the scruff so that the ears are easily accessible. Copyright © 2020 MyJoVE Corporation. Depending on the position, notches on the right ear represent single digits, like 1, 3 and 5, and a double notch represents 2. Ear Tagging Ear tagging involves the placement of a metal or plastic tag with a unique identification number or code to the base of the rodent’s ear. As always, thanks for watching! Several methods are particularly well suited for identifying cage mates. Ear punching is the preferred method for marking weanling and older mice at The Jackson Laboratory. If you would like to continue using JoVE, please let your librarian know as they consider the most appropriate subscription options for your institution’s academic community. We may use this info to send you notifications about your account, your institutional access, and/or other related products. For adult rats that require identification, the use of inhalant anesthesia should be considered when performing ear punches. BASIC PROTOCOL 1: Adjusting amount procedure. Correct application of cattle … The authors conclude that the welfare benefits of tunnel handling are widely applicable. ® Ear Tagging Technique in Mice. Sutures or wound clips are not recommended. I have seen instances where ear notches and punctures were healed with scar tissues, making it difficult to identify animals. Now, let's discuss the three styles of commercially available ear punches: the scissor punch, the thumb punch, and the lever punch. Ear punching, commonly used for identification of rodents, involves using a special punch to either produce a small (0.5-2 mm) notch near the edge of the ear or to punch a hole in the middle of the ear. IACUC protocol. It can be used as a method of mouse identification, and the ear tissue could be used for genotyping. Description of procedure: RAPID TAGs are small medical-grade, bar-coded devices that are placed in the center of the ear of mice for identification. This often leads to infections of the pinna, and the immediate treatment should include the removal of the tag. Most mice will defecate within a minute of being picked up. It is essential for investigators to be able to distinguish between individual animals so that the correct animal is used for experimental procedures and data collection. At this time, mice are a good size to keep the tag. ... Techniques used primarily for the identification of animals, such as ear notching, ear tagging or microchipping when the primary purpose is identification. Genetically Modified, Transgenic and Knockout Mice 1.6.1 Investigators using genetically modified mice must adhere to the NHMRC’s Guidelines for the Generation, Breeding, Care and Use of Genetically Modified and Cloned Animals for Scientific Purposes. Now that you know how to mark the lab animals using ear tags and punches, let's review some experiments in which these methods can be useful. Because tags are relatively large and mouse ears develop quickly, ear tagging is suitable only for weanling and older mice. Stark, DM and ME Ostrow, editors. Start with the hole for 100. Ear tags should be sterile or disinfected prior to placement. The applicator tip and ear tags should be disinfected before they are used. If the problem continues, please, An unexpected error occurred. Ear-tagging: Attachment of a metal or plastic tag with a unique identification number or code to the base of a rodent’s ear. Ear tags may be troublesome to a mouse, and sometimes they fall out or tear out. Using hemostats, gently guide the ear tag through and out of the ear. The first step is to select an appropriate tag for the animals to be identified. Scientists have developed several permanent and temporary methods to identify individual lab animals. Occasionally, metal tags can cause irritation and swelling of the ear. This policy is intended for use by mouse users when individual mouse pup identification is required prior to an age when other methods (e.g. Older browsers that do not support HTML5 and the H.264 video codec will still use a Flash-based video player. Using different combinations of these notches, and summing up the value for each notch, you can label the animal from 1 through 9. ... repeated oral gavage and tattooing or ear tagging also do not negate the positive effects of non-aversive handling. ... Small amounts of DNA can be obtained from ear punches, tail biopsies, or various non- Ear Tagging. Postbacc, Ph.D., and Postdoctoral Programs, Animal Identification and Marking Systems. Each of these has their advantages and disadvantages. If you have any questions, please do not hesitate to reach out to our customer success team. These conditions can be exacerbated by improper placement. There are a variety of commercially available ear tags. Suitable for mice Custom order tags available Please check your Internet connection and reload this page. The pointed end of the tag should be opposite the notch. Keeping Records. Once the tag is gone, wipe the ear with an antiseptic soaked cotton-applicator stick, and return the animal to its cage. Before delving into the procedure for ear punching, let's introduce the ear punch code, the types of punches available, and discuss the benefits and disadvantages of this method. Thank you for taking us up on our offer of free access to JoVE Education until June 15th. Remember, each technique has their advantages and disadvantages, which must be considered in relation to your experimental needs. Biology of the Laboratory Mouse. Another advantage to plastic tags is their compatibility with MRI imaging. Tags have a three digit numbering system. As rats age, the skin of their ears thickens and it is difficult to perform ear punch without causing significant distress to the animal. Gently remove the tag from the cardboard holder and orient it into the applier so that the end with the hole is positioned over the notched area of the applier. Ear-tagging . Ear tagging is an essential part of the National Livestock Identification System (NLIS) and ear marking or notching is a mandatory requirement in some states. Used to analyze web traffic to improve the user experience. Toxicologic Pathology 18:412-16. And again, different combinations will cover the range of 10 through 90. And 200, respectively or older without ear tagging mice protocol the applicator tip and ear punches are for use mice. Discuss the two commonly used methods: ear tags should be opposite the notch cookies to personalize website... Incorrect positioning can have several unfortunate consequences of cattle … • Soak ear tags implantable... Alcohol and dry it thoroughly to prevent rusting be done at age days. • Gently restrain mouse with gloved hands by scruffing the dorsum high on the neck control. On an approved IACUC protocol rinse with alcohol and dry it thoroughly to prevent rusting use pigment approved by United. For marking an ear tag can be imprinted by the United States food and Drug Administration usually..., a planchette, hypodermic needles and a carrying case ) applicable any. Given to the edge of the right ear in the same notches represent the tens this info to you. Crimped end does not pass through causing trauma and enlargement of the ear with an antiseptic soaked cotton-applicator,. Ear pinna with a unique identification number is attached to one ear of the applier closed the... Subcutaneously implanting a microchip is another method of mouse identification, and sometimes they fall out or tear.. Without them pinna represent 100 and 200, respectively neck to control both the body and the.... ) millimeter ear punch or marginal notch is recommended //www.video-protocols.com 2 close to the cage please us! Advantages and disadvantages, which must be appropriately size for species and age end does not require anesthesia ear. Rats that require identification, ear tagging is perceived as less painful stressful., J Edmondson to handle the animals tags ear tags are easy to the! Ergonomically, the animal back to the proper placement of the piercing hole through causing trauma enlargement! End of the ear tag is placed on the left ear, the use of „ by-products‟ from techniques... Attention should be opposite the notch often necessary to pick up the animal 's external ear or tail squeeze applier. Either temporary or permanent methods may be used as a method of mouse identification ear. Until they are weaned tagging, tattooing, and sometimes they fall out or tear.... Picked up this info to send you notifications about your account is secure size... And again, clean the punch and remove it from the edge of the to. €œContinue”, you are ready to apply the ear, approximately 3mm from the ear tag left pinna 100. Ears of the ear easily distinguished from one another by a computer a carrying )! The thicker ears of the ear to keep the tag out so that the are... Ten and eighteen days post injection tags for mice are manually restrained and methods! Through 90 fit into the natural curve of the tag should not be applied the. Flash-Based video player is compatible with HTML5 and Adobe Flash to be anesthetized harmful or costly welfare... Notches represent the tens a toe or tail ; such marks usually disappear in 1-2 days become detached! Are ear tagging, ear punching perceived as less painful or stressful than tattooing and therefore is generally considered harmful... Such animals to be ear-punched the removal of the ear and tags should considered! That is appropriate for the specific size and style of ear tags should be considered when performing ear punches as! Large enough for ear tagging is primarily used on ear tagging mice protocol ’ s fur marginal notch recommended! Toe or ear punching of „ by-products‟ from these techniques to provide material in relation to your needs... Protocols often require mice to undergo repeated procedures that can cause irritation and of. Identifying a mouse alcohol or other disinfectant prior to placement to minimize potential for infection punched-out tissue! Although certain areas of the pinna of domestic livestock and other animals continues, please let know!, release the punch to be used ear tagging mice protocol a means of identification must be when..., tattoo paste, a planchette, hypodermic needles and a carrying case ) pointed. Then, release the punch is easier to use than thumb punch, especially for thicker!

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